Histocompatibility Laboratories

• Detection and identification of HLA antibodies in sensitized patients and donors
• Determination of HLA types of patients and blood donors to assist in the provision of HLA-matched platelet transfusions
• HLA testing to support HCT and organ transplantation, including monitoring of engraftment post-HCT transplantation
• Identification of disease predisposition HLA gene for Celiac disease, narcolepsy, Ankylosing spondylitis and others
• Identification of drug hypersensitivity related HLA genes, including: –HLA-B*5701 with Abacavir –HLA-B*1502 with Carbamazepine –HLA-B*5801 with Allopurinol and others

Red Cross HLA Laboratories are ASHI and CLIA certified and accredited by various state specific licensures. HLA services include:

• HLA Typing
  • HLA Genes: HLA-A, -B, -C, -DRB1, -DRB3, -DRB4, -DRB5, -DQA1, -DQB1, -DPA1 and -DPB1
  • Resolution: Low, Intermediate, and High
    
  • Throughput: Low, High
• HLA Antibody Analysis
• T and B Cell Crossmatching
• Disease and Drug Sensitivity Association Study
• Platelet Antibody Screening
• Platelet Crossmatching
• HLA Matched Platelet for Transfusion
  
• Chimerism/Engraftment Monitoring
  • Whole Blood
  • Whole Marrow
  • CD3
  • CD19/20 B Cell
  • CD33/66 Myeloid Cell
  • CD56 NK Cell
• KIR Typing
• Relationship (Paternity) testing
• Identical Twin Verification
• CCR5 Delta 32 Mutation Test
• Additional services available

• HLA typing: Next Generation Sequencing (NGS), Sanger SBT, PCR-SSP, PCR-Sequence Specific Oligonucleotide Probeis (SSOP)
• HLA antibody detection/identification: Luminex Multi-Antigen Bead, Luminex Single Antigen Bead (SAB), Flow XM.
• Engraftment monitoring
• Disease/Drug sensitivity association: NGS, Sanger SBT, PCR-SSP, PCR-SSOP
• KIR Typing: Sanger SBT, PCR-SSP, PCR-SSOP
• Platelet Alloimmunization Screen/Crossmatch: Solid Phase Red Cell Adherence Assay (SPRCA)
• T and B Cell Crossmatching: Flow XM