HEA Genotyping Panel

Test Description

Allows for best matched red cell blood product for the patient and the avoidance of alloimmunization.

Test Type

Molecular

Additional Test Name(s)

Human Erythocyte Antigen (HEA) Genotyping Panel

Red Cell/RBC Genotyping Panel

Molecular RBC Typing

Sample Notes

All samples must be clearly labeled with the full name of the individual and a unique identification number. The information on the tube must match the information on the request form. Sample labels should also include date and time of collection. Samples less than 10 days old are preferred.

Whole Blood

• 5-10 mL whole blood in EDTA (lavender) tube
• If submitting pre- and post-transplant samples, clearly label them as such and submit separate service request.

Amniocytes

• 1 – 5 mL amniotic fluid or 1 – 5 x 10⁶ cultured amniocytes
• A maternal blood sample is required when submitting fetal sample. Submit with separate service request forms.
• Microsatellite analysis is performed on all maternal and amniocyte submissions to rule out maternal cell contamination (MCC).
• If MCC is detected, testing will not be performed.

Buccal Swabs

• Use sterile cotton-tipped applicator.
• Air dry swab before shipment in sterile tube without media.

Genomic DNA

• 20 - 25 ng/µL in microtube is required for testing
• Call for information.

Restrictions

• All Samples
  • Samples without sufficient information for unique identification will be rejected.
  • DNA yield of older specimen may be low or QNS and may not provide valid results.
  • Samples older than 10 days may be rejected.
• Whole Blood Samples Only
  • Lithium heparin sample tubes are NOT acceptable for testing.
  • Leukoreduced samples DO NOT yield acceptable DNA quantities for testing.
     

Requested Volume

Whole Blood: 5-10 mL

Shipping Information
 

• Whole blood samples can be shipped at room temperature or refrigerated using ice packs or wet ice sealed in plastic bags, according to DOT regulations for biological specimens.
• DNA, amniocytes, and buccal swabs MUST be shipped refrigerated using ice packs or wet ice sealed in plastic bags, according to DOT regulations for biological specimens.
• Wrap samples in absorbent materials to safeguard from freezing or breaking.
• Ship all samples "Next Day" delivery.

Shipping Address:
American Red Cross
National Molecular Laboratory
700 Spring Garden Street
Philadelphia, PA 19123

Shipping Label can be printed from the Connect Service order.

Method

Multiplex PCR and on bead elongation for fluoresence detection of single nucleotide variants.

Turnaround Time

5 business days

Accepts NY State Samples

Philadelphia